Journal
MOLECULAR ECOLOGY NOTES
Volume 5, Issue 2, Pages 282-288Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1471-8286.2005.00902.x
Keywords
cDNA; EST; expressed sequence tag; microsatellite; salmonidae; Salmo salar
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An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).
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