Journal
JOURNAL OF MASS SPECTROMETRY
Volume 40, Issue 6, Pages 777-784Publisher
WILEY
DOI: 10.1002/jms.852
Keywords
electrospray ionization mass spectrometry; noncovalent complexes; binding constant; beta-peptides; metal-peptide complexes
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We present an improvement of the titration method for binding constant determination with electrospray ionization (ESI) mass spectrometry that is unaffected by differences in ESI response of measured species in solution. The method consists of a calibration and titration, both using an internal standard that allows relative quantitation. This avoids artifacts such as a decrease in overall signal intensity with increasing ligand concentrations, rendering this approach more reliable and meaningful than direct evaluation of ESI peak intensities. We demonstrate the de novo binding constant determination of novel zinc binding beta-peptides, which have been synthesized with the goal of creating secondary structures stabilized by metal complexation. Copyright (c) 2005 John Wiley & Sons, Ltd.
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