Journal
SCIENCE
Volume 308, Issue 5727, Pages 1469-1472Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1108408
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Funding
- NIAMS NIH HHS [AR44420] Funding Source: Medline
- NIGMS NIH HHS [GM 068625, GM52111] Funding Source: Medline
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We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to, determine the average step size to be similar to 8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.
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