Journal
FEBS LETTERS
Volume 579, Issue 14, Pages 3119-3125Publisher
WILEY
DOI: 10.1016/j.febslet.2005.04.071
Keywords
hepatic stellate cell; peroxynitrite; matrix metalloproteinase; nitric oxide
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To investigate whether hepatic stellate cells (HSCs) alter their expression of MMPs after exposure to nitrogen oxide intermediate (NOI), a human hepatic stellate cell line, LI90 cells, was stimulated with an NO donor, SNAP, or a peroxynitrite donor, SIN-1, and the culture supernatants were analyzed by gelatin zymography or anti-MMPs immunoblot. Although SIN-1 did not enhance the secretions of MMP-1 and 13, SIN-1 induced the NF-kappa B activation, MT1-MMP expression and the secretion of activated MMP-2 in LI90 cells. These results suggest that peroxynitrite may contribute to the remodeling of the extracellular matrix in liver by activating pro-MMP-2. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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