Journal
JOURNAL OF CELL BIOLOGY
Volume 169, Issue 5, Pages 777-787Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200501104
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Funding
- NCRR NIH HHS [1S10RR015682] Funding Source: Medline
- NEI NIH HHS [EY10291, R01 EY010291] Funding Source: Medline
- NHLBI NIH HHS [P01 HL060678, T32HL07751, HL60678, T32 HL007751] Funding Source: Medline
- NIGMS NIH HHS [GM55101, R01 GM055101] Funding Source: Medline
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G alpha(12/ 13) have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both G alpha(12) and G alpha(13) signaling by overexpressing dominant negative proteins and application of antisense morpholinomodified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that G alpha(12/13) are required for cell elongation and efficient dorsalward migration during convergence independent of noncanonical Wnt signaling. Furthermore, G alpha(12/13) function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to noncanonical Wnt signaling. These findings provide the first evidence that G alpha(12) and G alpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.
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