Journal
FEBS LETTERS
Volume 579, Issue 14, Pages 3090-3094Publisher
WILEY
DOI: 10.1016/j.febslet.2005.04.067
Keywords
apoptosis; BAD; cell cycle; taxol
Funding
- NINDS NIH HHS [NS41021] Funding Source: Medline
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Phosphorylation of BCL-2 family member BAD at different residues triggers different physiological effects, either inhibiting or promoting apoptosis. The recently identified phosphorylation site at Ser-128 enhances the apoptotic activity of BAD. We here show that BAD becomes phosphorylated at Ser-128 in the mitotic phase of the cell cycle in NIH3T3 cells. We also show that BAD-S128 is phosphorylated in taxol-treated mouse fibroblasts and MDA-MB-231 human breast cancer cells. However, expression of a phosphorylation-defective dominant negative BAD mutant did not block taxol-induced apoptosis. These data support the view that the phosphorylation of BAD Serine 128 exerts cell-specific effects on apoptosis. Whereas the BAD Serine 128 phosphorylation induces apoptosis in neuronal cells, it does not appear to promote apoptosis in proliferating non-neural cells during mitosis or upon exposure to the antineoplastic agent taxol. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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