Journal
CELL
Volume 121, Issue 6, Pages 899-911Publisher
CELL PRESS
DOI: 10.1016/j.cell.2005.04.013
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Funding
- NIGMS NIH HHS [R37 GM025326, GM20627, F32 GM020627-01, R01 GM025326, GM25326, F32 GM020627, F32 GM020627-02, R01 GM025326-31] Funding Source: Medline
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Chromosome and replisome dynamics were examined in synchronized E. coli cells undergoing a eukaryotic-like cell cycle. Sister chromosomes remain tightly colocalized for much of S phase and then separate, in a single coordinate transition. Origin and terminus regions behave differently, as functionally independent domains. During separation, sister loci move far apart and the nucleoid becomes bilobed. Origins and terminus regions also move. We infer that sisters are initially linked and that loss of cohesion triggers global chromosome reorganization. This reorganization creates the 2-fold symmetric, ter-inlori-out conformation which, for E. coh, comprises sister segregation. Analogies with eukaryotic prometaphase suggest that this could be a primordial segregation mechanism to which microtubule-based processes were later added. We see no long-lived replication factory; replication initiation timing does not covary with cell mass, and we identify changes in nucleoid position and state that are tightly linked to cell division. We propose that cell division licenses the next round of replication initiation via these changes.
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