Journal
ANALYTICA CHIMICA ACTA
Volume 542, Issue 1, Pages 66-75Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2005.01.069
Keywords
molecularly imprinted microgels; phosphate; catalytic polymers
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Novel molecularly imprinted microgels incorporating arginine and tyrosine side chains as functional monomers have been designed and synthesised with percentages of cross-linker ranging from 70 to 90%. Full chemico-physical characterisation including M-r, coil density and size particle determination concluded that all polymer preparations obtained can be classified as microgels. Molecular imprinting using a phosphate template was used to generate catalytic microgels for the hydrolysis of p-nitrophenyl carbonates. Kinetic characterisation of the catalytic activity of the different preparations indicated that values of critical monomer concentration (Cm) and percentage of cross-linker play an important role in determining the catalytic efficiency of the different preparations. Microgels containing 70% cross-linker were the only ones following the Michaelis-Menten saturation model and kinetic parameters were obtained using 4 mg/ml of M397: V-max = 1.34 x 10(-6) M s(-1) (S.E. 1.28 x 10(-7)) and K-M = 2.38 x 10(-3) M (S.E. 3.1 x 10(-4)). (c) 2005 Elsevier B.V. All rights reserved.
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