Inhibition of gastric cancer-associated angiogenesis by antisense COX-2 transfectants

We characterized the effects of selective Cox-2 inhibition on the angiogenesis gastric cancer cell line SGC7901 by stable transfection of antisense Cox-2 cDNA (Cox-2-AS) or pharmacological inhibition by selective cox-2 inhibitor (NS398). The conditioned media obtained from SGC7901 treated with Cox-2-AS or NS398 suppressed the proliferation, migration and tube formation of human umbilical vein endothelial cells. Moreover, both treatments inhibited in vivo angiogenesis. These inhibitions could be partially reversed by the addition of PGE(2). Our findings support that selective inhibition of Cox-2 alone plays an instrumental role on gastric cancer associated angiogenesis. (c) 2004 Elsevier Ireland Ltd. All rights reserved.