Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1712, Issue 2, Pages 128-136Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2005.03.015
Keywords
3-hydroxyflavone derivative; cell plasma membrane; confocal fluorescence microscopy; dipole potential; fluorescence probe; fluorescence spectroscopy
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The dipole potential (Psi(d)) constitutes a large and functionally important part of the electrostatic potential of cell plasma membranes. However, its direct measurement is not possible. Herein, new 3-hydroxyflavone fluorescent probes were developed that respond strongly to Psi(d) changes by a variation of the intensity ratio of their two well-separated fluorescence bands. Using fluorescence spectroscopy with cell suspensions and confocal microscopy with adherent cells, we showed, for the first time, two-color fluorescence ratiometric measurement and visualization of Psi(d) in cell plasma membranes. Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced. (c) 2005 Elsevier B.V. All rights reserved.
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