Journal
JOURNAL OF VIROLOGY
Volume 79, Issue 13, Pages 8625-8628Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.79.13.8625-8628.2005
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Funding
- NCI NIH HHS [R01 CA108468] Funding Source: Medline
- NIGMS NIH HHS [R01 GM60562, R01 GM060562, P20 GM072069] Funding Source: Medline
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Respiratory syncytial virus (RSV) mediates serious lower respiratory tract illness in infants and young children and is a significant pathogen of the elderly and immune compromised. Rapid and sensitive RSV diagnosis is important to infection control and efforts to develop antiviral drugs. Current RSV detection methods are limited by sensitivity and/or time required for detection. In this study, we show that antibody-conjugated nanoparticles rapidly and sensitively detect RSV and estimate relative levels of surface protein expression. A major development is use of dual-color quantum dots or fluorescence energy transfer nanobeads that can be simultaneously excited with a single light source.
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