Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 382, Issue 5, Pages 1265-1268Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-005-3249-6
Keywords
rofecoxib; HPLC; serum; UV detection
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An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10-500 ng ml(-1).
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