Journal
INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY
Volume 20, Issue 2, Pages 174-177Publisher
SPRINGER INDIA
DOI: 10.1007/BF02867422
Keywords
C.S.F.protein; Modified Bromocresol green (BCG) method; Pyrogallol red method (Kit); SPSS 10.1 Statistical software
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Cerebrospinal fluid protein estimation is a routine diagnostic tool for various diseases. The proposed modified bromocresol green (BCG) method is applicable to automated as well as manual measurements. Protein concentrations and absorbance at 600 nm are linearly related throughout a wide range of concentrations from 10 mg % to 2000 mg %. Ninety C.S.F. samples were analyzed and results obtained by both the modified BCG method and routine pyrogallol red method were compared. An excellent co-relation was observed (n = 90, g = 0.996, P < 0.001).
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