Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 10, Pages 5907-5914Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b00122
Keywords
BODIPY; cysteine; homocysteine; glutathione; serum
Funding
- 973 program [2013CB933800]
- National Natural Science Foundation of China [21177125, 21321064, 21222210]
- Chinese Academy of Sciences
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Cysteine (Cys), homocysteine (Hey), and glutathione (GSH) are interconbected and play essential roles for regulating the redox balance of biological processes. However, finding a simple and effective method for the simultaneous determination for these three biothiols in biological systems is always a challenge. In this work, we report a method for the simultaneous quantitative determination of three biothiols in a mixture using a monochlorinated boron dipyrromethene (BODIPY)-based fluorometric sensor. At a specified period of time, after reacting with excess sensor, Hcy and GSH form predominantly sulfur-substituted BODIPY, while Cys generates sulfur-amino-diBODIPY due to a fast substitution-rearrangement substitution reaction. A significant difference in polarities of these respective major products simplifies their separation by TLC, thus leading to the simultaneous determination of Cys, Hey, and GSH readily. The sensor was successfully applied for the simultaneous quantitative detection of three biothiols in human serum, and the results were in good agreement with those obtained via high performance liquid chromatography (HPLC).
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