Journal
EXPERIMENTAL CELL RESEARCH
Volume 307, Issue 1, Pages 26-40Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2005.02.023
Keywords
beclin 1; Bcl-2 family; apoptosis; CDDP
Categories
Ask authors/readers for more resources
Beclin 1, identified as a Bcl-2-interacting protein, is known to enhance autophagy. However, the effect of Beclin I on apoptotic signaling has remained unclear. Here, we show that overexpression of Beclin 1 in MKN28 human gastric cancer cells augmented cis-diarnminedichloroplatinum (CDDP)-induced apoptosis. Conversely, knockdown of Beclin 1 by a small inhibitory RNA in MKN 1 cells attenuated this cytotoxicity. Furthermore, not only caspase-3/7 activities, but also caspase-9 activity was increased in Beclin I gene transfectants treated with CDDP, and caspase-9 inhibitor completely abolished augmentation of CDDP-induced apoptosis by Beclin I as did a caspase-3 inhibitor. Thus, Beclin 1 augments CDDP-induced apoptosis through enhancing caspase-9 activity and functions as a proapoptotic molecule. (c) 2005 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available