Journal
BIOTECHNOLOGY LETTERS
Volume 27, Issue 14, Pages 1025-1028Publisher
SPRINGER
DOI: 10.1007/s10529-005-8098-z
Keywords
bioreactor; gametophytes; macroalga; plasminogen activator protein; seaweed; transgenic kelp
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Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l(-1) was obtained in batch culture with an initial dry cell weight of 126 mg l(-1) and with aeration rate of 1.2 l air min(-1) l(-1) culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 mu g g(-1) dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.
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