The fluorescence characteristic of the yttrium-norfloxacin system and its analytical application

In this paper, a simple, rapid and sensitive spectrofluorimetric method based on the formation of yttrium complexes has been developed for the determination of norfloxacin (NFLX). The affecting factors of the enhancement were studied in detail. Under optimum conditions, a linear relationship was obtained between the enhanced fluorescence intensity and the NFLX concentration in the range 1.0 x 10(-9) to 1.0 x 10(-5) mol/l and the detection limit of NFLX was 3.8 x 10(-11) mol/l (S/N = 3). The method is applied for the determination of NFLX in actual sample (norfloxcain eye drops), the average recovery is 102.4% (n = 5), and the result obtained is satisfactory. (c) 2005 Elsevier B.V. All rights reserved.