Journal
TRENDS IN NEUROSCIENCES
Volume 28, Issue 7, Pages 343-345Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tins.2005.04.010
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Immunochemical analyses of protein expression and localization rely on the specificity of primary immunoreagents. A recent report, using transgenic mice, casts doubt on the specificity of three antibodies commonly used to immunolocalize alpha 7 nicotinic ACh receptors. These data highlight the conundrum facing histologists - how 'real' is the labelling they see?
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