Journal
CELL
Volume 121, Issue 7, Pages 1109-1121Publisher
CELL PRESS
DOI: 10.1016/j.cell.2005.05.026
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Funding
- NCI NIH HHS [CA46592] Funding Source: Medline
- NIAMS NIH HHS [1 P30 AR48310] Funding Source: Medline
- NICHD NIH HHS [R21 HD40760] Funding Source: Medline
- NIDDK NIH HHS [DK58771] Funding Source: Medline
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To improve our ability to identify hematopoletic stem cells (HSCs) and their localization in vivo, we compared the gene expression profiles of highly purified HSCs and non-self-renewing multipotent hematopoletic progenitors (MPPs). Cell surface receptors of the SLAM family, including CD150, CD244, and CD48, were differentially expressed among functionally distinct progenitors. HSCs were highly purified as CD150(+)CD244(-)CD48(-) cells while MPPs were CD244(+) CD150(-)CD48(-) and most restricted progenitors were CD48(+)CD244(+)CD150(-). The primitiveness of hematopoietic progenitors could thus be predicted based on the combination of SLAM family members they expressed. This is the first family of receptors whose combinatorial expression precisely distinguishes stem and progenitor cells. The ability to purify HSCs based on a simple combination of SLAM receptors allowed us to identify HSCs in tissue sections. Many HSCs were associated with sinusoidal endothelium in spleen and bone marrow, though some HSCs were associated with endosteum. HSCs thus occupy multiple niches, including sinusoldal endothelium in diverse tissues.
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