Journal
ANALYTICAL CHEMISTRY
Volume 77, Issue 13, Pages 4185-4193Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0481250
Keywords
-
Categories
Funding
- NIGMS NIH HHS [GM 061336] Funding Source: Medline
Ask authors/readers for more resources
The effect of esterification on MALDI ion yield is investigated by using alcohols having different aliphatic chain lengths. For peptides whose ionization yields increase with derivatization, more hydrophobic alcohols tend to yield greater peak enhancements. The completeness of the reaction increases from propanol to methanol. Undesired solvolysis of the amide group in the side chain of Asn or Gin leads to unexpected ester products. Ethanol is suggested as the optimal alcohol for esterification in proteomics experiments since it yields almost complete esterification without substantial solvolysis. Ethanol esterification was employed to facilitate the identification of gel-separated proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available