Journal
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
Volume 46, Issue 2, Pages 173-176Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S0003683810020080
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Funding
- Russian Foundation for Basic Research [07-04-12053]
- Ministry of Education and Science of the Russian Federation [2.1.1.605]
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It is shown that neutrophilic methylobacteria Methylophaga thalassica and M. marina have higher rates of growth and ectoin accumulation compared to the haloalkaliphilic species M. alcalica and M. natronica and methanotrophs Methylomicrobium alcaliphilum and M. kenyense. The conditions of M. thalassica cultivation in methanol-containing medium were optimized. The yield of this process reached 60 g/l of absolutely dry biomass containing 15-19% (9-11 g/l) ectoine. The scheme of ectoin isolation from the biomass by extraction and subsequent purification, which allowed obtaining preparations of different degree of purity, was developed.
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