Journal
ANALYTICAL CHEMISTRY
Volume 77, Issue 13, Pages 4081-4090Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac050222l
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Funding
- NIGMS NIH HHS [GM24349, R01 GM024349] Funding Source: Medline
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Silica-based lectin microcolumns are described in this study together with the chemical procedures necessary for their preparation. The analytical merits of Canavalia ensiformis and Sambucus nigra lectins, [immobilized on activated macroporous silica], such as binding capacity, trapping reproducibility, and substrate selectivity, have been evaluated using model glycoproteins. The described microcolumns are applicable to high-pressure analytical schemes utilizing microvalving procedures, washing steps, and quantitative desorption for LC/MS analysis. The described analytical systems are amenable to the applications aiming at fractionation of complex glycopeptide mixtures and determination of the sites of glycosylation.
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