Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 302, Issue 1-2, Pages 26-35Publisher
ELSEVIER
DOI: 10.1016/j.jim.2005.04.020
Keywords
myoglobin; cardiac markers; fluorescence immunoassay; metal-enhanced fluorescence; nano-size metallic particles; silver island films
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Funding
- NCRR NIH HHS [P41 RR008119, RR-08119] Funding Source: Medline
- NIBIB NIH HHS [R21 EB001690, R33 EB001690, EB-1690] Funding Source: Medline
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Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10-15-fold if compared to the signal from the glass substrate not modified with a SIR A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection. (c) 2005 Elsevier B.V. All rights reserved.
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