Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 173, Issue 7, Pages 1667-1679Publisher
SPRINGER
DOI: 10.1007/s12010-014-0954-7
Keywords
Microalgae; Heterotrophic cultivation; Oil accumulation; Algal biodiesel
Funding
- Shenzhen Development and Reform Commission [[2011] 835]
- Peking University Shenzhen Graduate School [0068]
- National Research Foundation and Economic Development Board of Singapore (SPORE) [COY-15-EWI-RCFSA/N197-1]
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This article presents a study on screening of microalgal strains from the Peking University Algae Collection and heterotrophic cultivation for biodiesel production of a selected microalgal strain. Among 89 strains, only five were capable of growing under heterotrophic conditions in liquid cultures and Chlorella sp. PKUAC 102 was found the best for the production of heterotrophic algal biodiesel. Composition of the growth medium was optimised using response surface methodology and optimised growth conditions were successfully used for cultivation of the strain in a fermentor. Conversion of algal lipids to fatty acid methyl esters (FAMEs) showed that the lipid profile of the heterotrophically cultivated Chlorella sp. PKUAC 102 contains fatty acids suitable for biodiesel production.
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