Journal
JOURNAL OF PROTEOME RESEARCH
Volume 4, Issue 4, Pages 1330-1338Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr050044c
Keywords
proteome analysis; beta-barrel analysis; Anabaena; outer membrane
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Anabaena is a model to analyze the evolutionary development of plastids, cell differentiation, and the regulation of nitrogen fixation. Thereby, the outer membrane proteome is the place of sensing environmental differences and during plastid development, systems for intracellular communication had to be added to the proteome of this membrane. We present a protocol for the isolation of the outer membrane from Anabaena and the analysis of the proteome using different tools. 55 Proteins were identified.
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