Journal
FREE RADICAL RESEARCH
Volume 39, Issue 7, Pages 787-795Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715760500045806
Keywords
comet assay; DNA damage; hydrogen peroxide; olive oil; olive mill waste water
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In search for compounds, able to protect nuclear DNA in cells exposed to oxidative stress, extracts from olive leaves, olive fruits, olive oil and olive mill waste water were tested by using the single cell gel electrophoresis methodology (comet assay). Jurkat cells in culture were exposed to continuously generated hydrogen peroxide (11.8 +/- 1.5 mu M per min) by direct addition into the growth medium of the appropriate amount of the enzyme glucose oxidase in the presence or absence of the tested total extracts. The protective effects of the tested extracts or isolated compounds were evaluated from their ability to decrease hydrogen peroxide-induced formation of single strand breaks in the nuclear DNA, while the toxic effects were estimated from the increase of DNA damage when the extracts or isolated compounds were incubated directly with the cells. Significant protection was observed in extracts from olive oil and olive mill waste water. However, above a concentration of 100 mu g/ml olive oil extracts exerted DNA damaging effects by themselves in the absence of any H2O2. Extracts from olive leaves and olive fruits although protective, were also able to induce DNA damage by themselves. Main compounds isolated from the above described total extracts, like oleuropein glucoside, tyrosol, hydroxytyrosol and caffeic acid, were tested in the same experimental system and found to exert cytotoxic (oleuropein glucoside), no effect (tyrosol) or protective effects (hydroxytyrosol and caffeic acid). In conclusion, cytoprotective as well as cytotoxic compounds with potential pharmaceutical properties were detected in extracts from olive oil related sources by using the comet assay methodology.
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