Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 169, Issue 4, Pages 1292-1305Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-012-0037-6
Keywords
Indole-3-acetic acid; Fusarium delphinoides; Phytopathogen; IAA biosynthetic pathway; Aminotransferase
Funding
- University Grants Commission (UGC), New Delhi, India through Major Research Project
- University Grants Commission (UGC), New Delhi, India through SAP programme
- Council of Scientific and Industrial Research (CSIR)
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Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysis of FDG culture filtrates showed the presence of metabolic intermediates of indole-3-pyruvic acid (IPyA), indole-3-acetamide (IAM), and tryptamine (TRA) pathways. The different IAA biosynthetic pathways were further confirmed by identifying the presence of different enzymes of these pathways. Substrate specificity study of aromatic amino acid aminotransferase revealed that the enzyme is highly specific for tryptophan (Trp) and alpha-ketoglutarate (alpha-kg) as amino group donor and acceptor, respectively. Furthermore, the concentration-dependent effect of exogenous IAA on fungal growth was established. Low concentration of exogenous IAA increases the fungal growth and at high concentration it decreases the growth of FDG.
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