Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 169, Issue 4, Pages 1315-1328Publisher
SPRINGER
DOI: 10.1007/s12010-012-0057-2
Keywords
Homogenization; Microcrystalline cellulose; Enzymatic hydrolysis; Crystallinity; Water retention
Funding
- Walloon Region in the frame of the NACELL program [031/5575]
- LIGNOFUEL program [716721]
- TECHNOSE program [716757]
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The aim of this study is to compare the effect of different homogenization treatments on the physicochemical properties and the hydrolysis rate of a pure bleached cellulose. Results obtained show that homogenization treatments improve the enzymatic hydrolysis rate of the cellulose fibers by 25 to 100 %, depending of the homogenization treatment applied. Characterization of the samples showed also that homogenization had an impact on some physicochemical properties of the cellulose. For moderate treatment intensities (pressure below 500 b and degree of homogenization below 25), an increase of water retention values (WRV) that correlated to the increase of the hydrolysis rate was highlighted. Result also showed that the overall crystallinity of the cellulose properties appeared not to be impacted by the homogenization treatment. For higher treatment intensities, homogenized cellulose samples developed a stable tridimentional network that contributes to decrease cellulase mobility and slowdown the hydrolysis process.
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