Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 171, Issue 6, Pages 1444-1453Publisher
SPRINGER
DOI: 10.1007/s12010-013-0441-6
Keywords
Lentinus edodes mycelia; Polysaccharide; Purification; Antitumor; Laryngeal carcinoma
Funding
- National Natural Science Foundation of China [31240005]
- Agriculture research Program of Science and Technology Department of Liaoning Province, China [2011211001, 2009209001]
- Program for Liaoning Excellent Talents in University [LJQ2013002]
- Startup Foundation for Doctors of Liaoning University
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A novel polysaccharide [Lentinus edodes mycelia polysaccharide 2 (LMP2)] with a molecular weight of 2.27 x 10(4) Da was isolated from the Lentinus edodes mycelia and purified by Sephadex G-200 and diethylaminoethyl-32 column chromatography. HPLC results indicated that LMP2 contained mannose, arabinose, galactose, xylose, and rhamnose with a relative molar ratio of 1:0.74:3.23:1.18:10.98. Its antitumor activity was evaluated in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and transwell assay. LMP2 showed a remarkable inhibitory effect on the proliferation of Hep-2 cells, and at the concentration of 200 mg/ml, the inhibition ratio was 37.2 % after 72 h. Furthermore, colony formation of Hep-2 cells was reduced significantly after treatment with LMP2. Invasion of Hep-2 cells was inhibited significantly by LMP2. These results suggested that LMP2 could be explored as a potential antitumor material for laryngeal carcinoma.
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