Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 171, Issue 3, Pages 555-568Publisher
SPRINGER
DOI: 10.1007/s12010-013-0414-9
Keywords
Butanol; Clostridium acetobutylicum; Xylose pathway engineering; Xylose mother liquor
Funding
- National Natural Science Foundation of China [31270140]
- Knowledge Innovation Program of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences [2012KIP509]
- Ministry of Science and Technology of China [2012BAD32B07, 2011AA02A208, 2012CB721105]
- Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-J-12, KSCX2-EW-G-13-5, KSCX2-EW-G-1]
- SA-SIBS Scholarship Program
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Xylose mother liquor (XML) is a by-product of xylose production through acid hydrolysis from corncobs, which can be used potentially for alternative fermentation feedstock. Sixteen Clostridia including 13 wild-type, 1 industrial strain, and 2 genetically engineered strains were screened in XML, among which the industrial strain Clostridium acetobutylicum EA 2018 showed the highest titer of solvents (12.7 g/L) among non-genetic populations, whereas only 40 % of the xylose was consumed. An engineered strain (2018glcG-TBA) obtained by combination of glcG disruption and expression of the d-xylose proton-symporter, d-xylose isomerase, and xylulokinase was able to completely utilize glucose and l-arabinose, and 88 % xylose in XML. The 2018glcG-TBA produced total solvents up to 21 g/L with a 50 % enhancement of total solvent yield (0.33 g/g sugar) compared to that of EA 2018 (0.21 g/g sugar) in XML. This XML-based acetone-butanol-ethanol fermentation using recombinant 2018glcG-TBA was estimated to be economically promising for future production of solvents.
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