4.4 Article

Enhancement of the Enzymatic Digestibility and Ethanol Production from Sugarcane Bagasse by Moderate Temperature-Dilute Ammonia Treatment

Journal

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 171, Issue 5, Pages 1108-1117

Publisher

SPRINGER
DOI: 10.1007/s12010-013-0327-7

Keywords

Dilute ammonia soaking pretreatment; Sugarcane bagasse; Moderate temperature; Ethanol

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In this study, sugarcane bagasse was pretreated with ammonium hydroxide, and the effectiveness of the pretreatment on enzyme hydrolysis and ethanol production was examined. Bagasse was soaked in ammonium hydroxide and water at a ratio of 1:0.5:8 for 0-4 days at 70 A degrees C. Approximately, 14-45 % lignin, 2-6 % cellulose, and 13-22 % hemicellulose were removed during a 0.5- to 4-day ammonia soaking period. The highest glucan conversion of sugarcane bagasse soaked in dilute ammonia at moderate temperature by cellulase was accomplished at 78 % with 75 % of the theoretical ethanol yield. Under the same conditions, untreated bagasse resulted in a cellulose digestibility of 29 and 27 % of the theoretical ethanol yield. The increased enzymatic digestibility and ethanol yields after dilute ammonia pretreatment was related to a combined effect of the removal of lignin and increase in the surface area of fibers.

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