Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 171, Issue 1, Pages 246-260Publisher
SPRINGER
DOI: 10.1007/s12010-013-0366-0
Keywords
Exiguobacterium sp VSG-1; Lignocellulolytic enzymes; Sugarcane bagasse (SCB); Enzymatic hydrolysis; Bioethanol
Funding
- DST
- UGC-SAP, the Government of India, New Delhi
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Exiguobacterium sp. VSG-1 was isolated from the soil sample and characterized for the production of lignocellulolytic enzymes. Production of these enzymes by the strain VSG-1 was carried out using steam-exploded sugarcane bagasse (SCB) and found to secrete cellulase, pectinase, mannanase, xylanase, and tannase. The growth and enzyme production were found to be optimum at pH 9.0 and 37 A degrees C. Upon steam explosion of SCB, the cellulose increased by 42 %, whereas hemicelluloses and lignin decreased by 40 and 62 %, respectively. Enzymatic hydrolysis of steam-exploded SCB yielded 640 g/l of total sugars. Fermentation of sugars produced from pretreated SCB was carried out by using Saccharomyces cerevisiae at pH 5.0 and 30 A degrees C. The alcohol produced was calculated and found to be 62.24 g/l corresponding to 78 % of the theoretical yield of ethanol. Hence, the strain VSG-1 has an industrial importance for the production of fermentable sugars for biofuels.
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