Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 172, Issue 3, Pages 1282-1292Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-013-0601-8
Keywords
Klebsiella pneumoniae; Bioflocculant; Thermostable; Polysaccharide
Funding
- Wuhan Textile University [2013018]
- China Postdoctoral Science Foundation [2013M541656]
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The production and characterization of a bioflocculant, MBF-6, by Klebsiella pneumoniae was investigated. Optimum culture conditions for bioflocculant production were an initial medium pH of 7, an incubation temperature of 30 degrees C, and an inoculum size of 1 % (v/v) of cell density 1.0x10(8) cfu/mL. The carbon, nitrogen, and cation sources for optimum bioflocculant production were glucose, peptone, and ZnCl2. The bioflocculant mainly consisted of protein (3.4 %) and sugar (95.1 %). Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl and hydroxyl groups while the thermogravimetric analysis (TGA) showed a degradation temperature (T-d) of 81.4 degrees C. MBF-6 had a good flocculating rate in kaolin suspension without cation addition and was stable over a wide range of pH and temperature. Investigation on the flocculation efficacy of the characterized MBF-6 for wastewater treatment of dairy, woolen, brewery, and sugar industries suggested it to be effective.
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