Journal
ACCOUNTS OF CHEMICAL RESEARCH
Volume 38, Issue 7, Pages 542-548Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ar040138c
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Funding
- NIGMS NIH HHS [T32GM008276, GM065367, R01 GM065367] Funding Source: Medline
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Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.
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