Journal
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 140, Issue 1, Pages 138-143Publisher
SPRINGER
DOI: 10.1007/s10517-005-0430-z
Keywords
mesenchymal stem cells; cell culture; bone marrow; lipoaspirate; differentiation; phenotype
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Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cyto-fluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD 117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
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