Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 165, Issue 7-8, Pages 1519-1531Publisher
SPRINGER
DOI: 10.1007/s12010-011-9372-2
Keywords
Phenotypically; Physiologically-biochemically; Phylogenetically; 16S rRNA genes; Gluconacetobacter hansenii subsp nov.; Genetic diversity
Funding
- Fundamental Research Funds for the Central Universities [QN2009072]
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Strain M-438, deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-producing strain ever reported. In this paper, M-438 was identidied as Gluconacetobacter hansenii subsp. nov. on the basis of the results obtained by examining it phylogenetically, phenotypically, and physiologically-biochemically. Furthermore, the genetic diversity of strain M-438 and its initial strain was examined by amplified fragment length polymorphism. The results indicated that strain M-438 was a deletion mutant induced by HHP, and the only deleted sequence showed 99% identity with 24,917-24,723 bp in the genome sequence of Ga. hansenii ATCC23769, and the complement gene sequence was at 24,699-25,019 bp with local tag GXY_15142, which codes small multidrug resistance (SMR) protein. It can be inferred that SMR might be related to inhibiting BC production to a certain extent.
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