Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 43, Issue 7, Pages 3304-3308Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.43.7.3304-3308.2005
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Given the emergence of drug resistance and the high rate of polyclonal microorganism infections, the availability of a fast and sensitive test to detect minority mutant populations would be an improvement in the diagnosis of infectious diseases. A clamped-probe real-time PCR assay to diagnose the Plasmodium falciparum K76T mutation in clone populations was developed, using a wild-type-specific locked-nucleic-acid-containing oligomer to suppress wild-type PCR amplification and to enhance melting analysis with a mutation-specific detection probe.
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