Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 166, Issue 2, Pages 259-267Publisher
SPRINGER
DOI: 10.1007/s12010-011-9421-x
Keywords
Ergothioneine; Histidine; Tyrosinase; DPPH
Funding
- National Science Council, Taiwan, ROC [NSC99-2622-B-241-003-CC3]
Ask authors/readers for more resources
The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC50 value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompetitive tyrosinase inhibitor using a Lineweaver-Burk plot of experimental kinetic data. The IC50 value for ergothioneine scavenging of 2,2-diphenyl-1-picrylhydrazyl was 6.110+/-0.305 mg/ml, much higher than the IC50 for inhibition of tyrosinase activity which indicating ergothioneine on tyrosinase shows a weak correlation to its antioxidative activity. The results demonstrated that ergothioneine has a potent inhibition effect on tyrosinase enzyme activity, resulting from the presence of the sulfur substituted imidazole ring in ergothioneine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available