Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 162, Issue 7, Pages 1847-1857Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-010-8962-8
Keywords
Cellulosic ethanol; Palm fiber; AFEX; Saccharomyces cerevisiae; Biofuel; Pretreatment
Funding
- DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science) [DE-FC02-07ER64494]
Ask authors/readers for more resources
Empty palm fruit bunch fiber (EPFBF), a readily available cellulosic biomass from palm processing facilities, is investigated as a potential carbohydrate source for cellulosic ethanol production. This feedstock was pretreated using ammonia fiber expansion (AFEX) and enzymatically hydrolyzed. The best tested AFEX conditions were at 135 A degrees C, 45 min retention time, water to dry biomass loading of 1:1 (weight ratio), and ammonia to dry biomass loading of 1:1 (weight ratio). The particle size of the pretreated biomass was reduced post-AFEX. The optimized enzyme formulation consists of Accellerase (84 mu L/g biomass), Multifect Xylanase (31 mu L/g biomass), and Multifect Pectinase (24 mu L/g biomass). This mixture achieved close to 90% of the total maximum yield within 72 h of enzymatic hydrolysis. Fermentation on the water extract of this biomass affirms that nutrients solely from the pretreated EPFBF can support yeast growth for complete glucose fermentation. These results suggest that AFEX-treated EPFBF can be used for cellulosic biofuels production because biomass recalcitrance has been overcome without reducing the fermentability of the pretreated materials.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available