4.4 Article

Cellulases and Xylanases Production by Penicillium echinulatum Grown on Sugar Cane Bagasse in Solid-State Fermentation

Journal

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 162, Issue 7, Pages 1889-1900

Publisher

HUMANA PRESS INC
DOI: 10.1007/s12010-010-8967-3

Keywords

Solid-state fermentation; Cellulases; Xylanases; Sugar cane bagasse; Second-generation ethanol

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To investigate the production of cellulases and xylanases from Penicillium echinulatum 9A02S1, solid-state fermentation (SSF) was performed by using different ratios of sugar cane bagasse (SCB) and wheat bran (WB). The greatest filter paper activity obtained was 45.82 +/- 1.88 U gdm(-1) in a culture containing 6SCB/4WB on the third day. The greatest beta-glucosidase activities were 40.13 +/- 5.10 U gdm(-1) obtained on the third day for the 0SCB/10WB culture and 29.17 +/- 1.06 U gdm(-1) for the 2SCB/8WB culture. For endoglucanase, the greatest activities were 290.47 +/- 43.57 and 276.84 +/- 15.47 U gdm(-1), for the culture 6SCB/4WB on the fourth and fifth days of cultivation, respectively. The greatest xylanase activities were found on the third day for the cultures 6SCB/4WB (36.38 +/- 5.38 U gdm(-1)) and 4SCB/6WB (37.87 +/- 2.26 U gdm(-1)). In conclusion, the results presented in this article showed that it was possible to obtain large amounts of cellulases and xylanases enzymes using low-cost substrates, such as SCB and WB.

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