Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 162, Issue 5, Pages 1403-1413Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-010-8916-1
Keywords
Ganoderma sinensis; Fungal immunomodulatory protein; Expression; Cytokine
Funding
- National Natural Science Foundation of China [30771500]
- Shanghai Science and Technology Committee [05DZ05007]
- Shanghai Leading Academic Discipline Project [B209]
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Ganoderma sinensis fungal immunomodulatory protein (FIP-gsi) was a new member of FIPs family. Based on the cloning of FIP-gsi gene from G. sinensis, this paper reported that FIP-gsi gene was expressed in Escherichia coli expression system. Then, the recombinant proteins were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Finally, the immunomodulatory activity was examined by inducing cytokine genes expression. The results showed that the recombinant FIP-gsi protein could be expressed in E. coli and got the yield of about 25% of the soluble form in the total soluble protein. The FIP-gsi protein was composed of 111 amino acids, and the sequence of homologous rate was 88.6% with FIP-glu (LZ-8). Furthermore, it could enhance the levels of interleukin (IL)-2, IL-3, IL-4, interferon gamma, tumor necrosis factor alpha, and IL-2 receptor (IL-2R) in mouse spleen cells.
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