Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 162, Issue 5, Pages 1446-1456Publisher
SPRINGER
DOI: 10.1007/s12010-010-8919-y
Keywords
Mycobacterium neoaurum; Extraction chromatography; Screening; Phytosterols; 4-Androstene-3,17-dione (AD); 1,4-Androstadiene-3,17-dione (ADD); 3-Ketosteroid 9 alpha-hydroxylase (KSH)
Funding
- National Basic Research Program of China [2009CB724703]
- National High Technology Research and Development Program of China [2008AA02Z209]
Ask authors/readers for more resources
Using enrichment procedures, five strains that can utilize soybean phytosterols as the sole carbon source were isolated from steroids-contaminated soil samples. Among the isolated strains, the strain NwIB-01 with the highest steroid degradation ability was identified as Mycobacterium neoaurum by morphological, physiological, biochemical tests and 16S rRNA sequence analysis. Meanwhile, the key enzyme gene, which was involved in steroid metabolism and encoding 395-amino acid 3-ketosteroid 9 alpha-hydroxylase (KSH), was obtained from M. neoaurum NwIB-01 with the assistance of homology analysis and chromosome walking. To our best knowledge, this is the first report to the gene of key enzyme KSH from M. neoaurum. Strain NwIB-01 exhibited powerful ability of cleaving the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD). It was showed that when cultured in 15 g/l phytosterols, the yield of ADD reached 4.23 g/l while accompanied by 1.76 g/l AD in 96-h-old culture (the molar yield of AD + ADD is 64.7%). The strain NwIB-01 can be applied as excellent phytosterols-transformation strains in potential industrial applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available