Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 160, Issue 5, Pages 1332-1340Publisher
SPRINGER
DOI: 10.1007/s12010-009-8681-1
Keywords
Bacillus licheniformis; Halotolerant; Poly-gamma-glutamic acid; Molecular size; Yield
Funding
- Program for New Century Excellent Talents in University of China [NCET-07-0341]
- National High-Tech Research and Development Program of China [2008AA10Z317]
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A poly-gamma-glutamic acid (gamma-PGA) productive strain, halotolerant bacterium WX-02 was isolated from the saline soil of China (Yingcheng). By physiological, biochemical, and 16S rDNA sequence analysis methods, the strain was identified as Bacillus licheniformis. The effect of NaCl concentration on gamma-PGA production by WX-02 was investigated in modified E (ME) medium. It was found that the gamma-PGA production was salt-inducible, and the highest volumetric yield of gamma-PGA (13.86 g/l) was attained with 8% of NaCl. It was also observed that the molecular size of gamma-PGA decreased when the NaCl concentration increased. This was the first report of isolation and identification of a gamma-PGA productive strain, halotolerant B. licheniformis. This study provided a simple strategy for controlling the yield and molecular size of gamma-PGA by WX-02.
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