Journal
ANNALS OF NEUROLOGY
Volume 58, Issue 1, Pages 147-150Publisher
WILEY
DOI: 10.1002/ana.20524
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Funding
- NIA NIH HHS [AG09464] Funding Source: Medline
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Amyloid-beta (A beta) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of A beta results in underestimated A beta ELISA levels. The implications are that comprehensive analysis of soluble A beta requires either sample pretreatment at denaturing conditions or novel conformation-dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species.
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