Journal
FEMS YEAST RESEARCH
Volume 5, Issue 10, Pages 935-942Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsyr.2005.03.009
Keywords
yeast; heterologous gene expression; expression vector; selectable marker; MET2
Funding
- NIGMS NIH HHS [GM65882] Funding Source: Medline
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We describe the isolation and characterization of a new biosynthetic gene, MET2, from the methylotrophic yeast Pichia pastoris. The predicted product of PpMET2 is significantly similar to its Saccharomyces cerevisiae counterpart, SeMET2, which encodes homoserine-O-transacetylase. The ScMET2 was able to complement the P. pastoris met2 strain; however, the converse was not true. Expression vectors based on PpMET2 for the intracellular and secreted production of foreign proteins and corresponding auxotrophic strains were constructed and tested for use in heterologous expression. The expression vectors and corresponding strains provide greater flexibility when using P. pastoris for recombinant protein expression. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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