Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 160, Issue 2, Pages 370-377Publisher
SPRINGER
DOI: 10.1007/s12010-008-8307-z
Keywords
Poly-gamma-glutamic acid; L-glutamic acid; Bacillus subtilis; Cultivation conditions; Optimization; Scale up
Funding
- National High Technology Research and Development Program of China [2006AA02Z239]
- National Basic Research Program of China [2007CB707805]
- Ministry of Science and Technology, China
- National Science Foundation of China [20736008, 20676115]
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Poly-gamma-glutamic acid (gamma-PGA) is a kind of water-soluble and biodegradable polymer made from d- and l-glutamic acid units, which are linked by amide bonds formed between alpha-amino and gamma-carboxylic acid groups. As a potential targeted biopolymer that can be refined from biomass directly, gamma-PGA has been increasingly applied to food, cosmetic, and pharmaceutical industries. In this work, a suitable nitrogen source was screened out for the high and cost-effective production of gamma-PGA in Bacillus subtilis ZJU-7. The effects of inoculation time and initial glucose concentration on the gamma-PGA production were investigated systematically in both shake flasks and a bench-top 15-l fermentor. Under the optimized culture conditions, a high gamma-PGA productivity (46.4 g/l) was obtained after 48 h cultivation at 37 A degrees C. Finally, the large-scale fermentation of gamma-PGA production was successfully scaled up to a 100-l fermentor, with the highest gamma-PGA productivity for over 54.0 g/l.
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