Journal
REPRODUCTIVE TOXICOLOGY
Volume 20, Issue 2, Pages 183-193Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2005.01.009
Keywords
in vitro follicle bioassay; diazepam; folliculogenesis; steroidogenesis; oocyte quality; nuclear maturation; spindle/chromosome organization
Categories
Ask authors/readers for more resources
A mouse ovarian follicle bioassay was used to study folliculogenesis and oocyte quality in vitro. Diazepam (DZ) was chosen as test compound to evaluate the system for its ability to detect possible effects of chemicals on reproduction. The bioassay is suitable to analyze the influence of DZ on each of the follicular components at any stage of development. A dose finding study revealed that follicle growth, differentiation and steroidogenesis were significantly disturbed by >= 5 mu g/ml DZ. A transient exposure procedure was used to examine stage-specific sensitivities of oogenesis to DZ. The oocyte appeared to be most vulnerable during its growth process within the follicle. Resumption of meiosis was disturbed dose-dependently with reduced oocyte quality after chronic exposure to >= 2.5 mu g/ml DZ. The bioassay is a highly efficient and informative tool to assess the hazards of chemical compounds for female fertility and to elucidate their mechanisms of action. (c) 2005 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available