Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 158, Issue 2, Pages 243-252Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-008-8332-y
Keywords
Post-transcriptional gene silencing (PTGS); Suppressor; P1/HC-Pro; Agrobacterium-mediated transient expression system; GFP; Semi-quantitative reverse transcription-PCR
Funding
- Program for Changjiang Scholars and Innovative Research Team (PCSIRT) [IRT0519]
- Programme of Introducing Talents of Discipline [B07017]
- Chinese National Plant Transformation Center [JY03-B-17]
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More than 20 post-transcriptional gene silencing (PTGS) suppressors have been found since HC-Pro, the first gene silencing suppressor, was found in 1998. The silencing suppressor strongly suggested that gene silencing functions as natural defense mechanisms against viruses. It also represented a valuable tool for the dissection of the gene silencing pathway. We have used P1/HC-Pro RNA silencing suppressor activity to increase green fluorescent protein (GFP) expression in tobacco using an Agrobacterium-mediated transient expression system. P1/HC-Pro stimulated GFP-gene expression but not dsGFP-gene expression was shown by RT-PCR, Northern and Western blot analysis. Expression of the gene silencing suppressor and the target gene provided a new strategy of heterogeneous gene expressing in plants. It may be of commercial significance to produce foreign proteins using plant bioreactors.
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