Increased specific T cell cytokine responses in patients with active pulmonary tuberculosis from Central Africa

An understanding of T cell responses that are crucial for control of Mycobacterium tuberculosis (MTB) has major implications for the development of immune-based interventions. We studied the frequency of purified protein derivative (PPD)-specific CD3(+) cells expressing interleukin-2 (IL)-2, gamma interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha and IL-10 in HIV-negative pulmonary tuberculosis patients (TB, n = 30) as well as in healthy individuals (controls, n = 21) from Central Africa. Increased frequencies of PPD-stimulated CD3(+) cells expressing IL-2, IFN-gamma, and TNF-alpha in TB were seen when compared with frequencies of controls. The presence of type 1 cytokine biased responses in TB patients was supported by a shift in the distribution pattern of cytokine expression from exclusively IL-2 or TNF-alpha expression seen in controls towards an increased frequency of IFN-gamma/IL-2 or IFN-gamma/TNF-alpha co-expression in TB. Higher levels of PPD-induced IFN-gamma in the supernatants from TB patients than from controls were found, which correlated with its intracellular expression. PPD was a weak inducer of IL-10 in T cells and insufficient in promoting cytokine production in TCR gamma delta(+)CD3(+) cells. Non-specific stimulation with PMA and iono-mycin revealed increased frequencies of CD4(+) cells expressing IFN-gamma in controls, while expression of IL-2, IL-4, IL- 10, IL- 13, and TNF-alpha was not different. Non-specific cytokine responses of TCR gamma delta(+)CD3(+) cells were similar in all groups. pulmonary TB in Central Africa is associated with enhanced expression and secretion of specifically induced cytokines that are frequently implicated in host defense against MTB. (C) 2005 Elsevier SAS. All rights reserved.