Journal
CELLULAR AND MOLECULAR LIFE SCIENCES
Volume 62, Issue 13, Pages 1514-1525Publisher
BIRKHAUSER VERLAG AG
DOI: 10.1007/s00018-005-5099-y
Keywords
procoagulant activity; scramblase; aminophospholipid translocase; thrombin; collagen; platelet; phosphatidylserine; microparticle
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Platelet procoagulant activity is mainly determined by the extent of surface-exposed phosphatidylserine (PS), controlled by the activity of aminophospholipid translocase and phospholipid scramblase. Here, we studied both transport activities in single platelets upon stimulation with various agonists. Besides the formation of procoagulant microparticles, the results show that a distinct fraction of the platelets exposes PS when stimulated. The extent of PS exposure in these platelet fractions was similar to that in platelets challenged with Ca(2+)-ionophore, where all cells exhibit maximal attainable PS exposure. The size of the PS-exposing fraction depends on the agonist and is proportional to the platelet procoagulant activity Scramblase activity was observed only in the PS-exposing platelet fraction, whereas translocase activity was exclusively detectable in the fraction that did not expose PS. We conclude that, irrespective of the agonist, procoagulant platelets exhibit maximal surface exposure of PS by switching on scramblase and inhibiting translocase activity.
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